Ontology highlight
ABSTRACT:
SUBMITTER: Kang T
PROVIDER: S-EPMC7554026 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Kang Taek T Quarton Tyler T Nowak Chance M CM Ehrhardt Kristina K Singh Abhyudai A Li Yi Y Bleris Leonidas L
iScience 20200921 10
MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression post-transcriptionally by binding to target messenger RNAs (mRNAs). Many human miRNAs are intragenic, located within introns of protein-coding sequence (host). Intriguingly, a percentage of intragenic miRNAs downregulate the host transcript forming an incoherent feedforward motif topology. Here, we study intragenic miRNA-mediated host gene regulation using a synthetic gene circuit stably integrated within a safe- ...[more]