GHR^-/- Mice are protected from obesity-related white adipose tissue inflammation.

ABSTRACT: Growth hormone (GH) excess in bovine (b)GH transgenic mice has been shown to alter white adipose tissue (WAT) immune cell populations. The present study aimed to evaluate the effects of GH resistance on WAT immune cell populations using GH receptor knockout (GHR^-/- ) mice. Eight- and 24-month-old, male GHR^-/- and wild-type mice were used. Body composition and tissue weights were determined, and systemic inflammation was assessed by measuring serum cytokine levels. The stromal vascular fraction (SVF) was isolated from three distinct WAT depots, and immune cell populations were quantified using flow cytometry. GHR^-/- mice at both ages had decreased body weight but were obese. Although no significant changes were observed in serum levels of the measured cytoki

SUBMITTER: Young JA 

PROVIDER: S-EPMC7554100 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature