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Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease.

ABSTRACT: The cytoplasmic lipid droplet (CLD) protein perilipin-2 (Plin2) is expressed in multiple nonadipose tissues, where it is thought to play a role in regulating their lipid storage properties. However, the extent to which Plin2 functions in nutrient utilization and metabolism, or how it influences the consequences of over-feeding, remains unclear. In this study, we demonstrate that the absence of Plin2 prevents high-fat diet(HFD)-induced obesity in male and female mice. This response is associated with increased formation of subcutaneous beige adipocyte cells with uncoupling protein 1 expression, and amelioration of inflammatory foci formation in white adipose tissue and steatosis in the liver. Experiments demonstrate that Plin2 loss results in reduced energy intake and increased physical activity in response to HFD feeding. Our study provides the first evidence that Plin2 contributes to HFD-induced obesity by modulating food intake, and that its absence prevents obesity-associated adipose tissue inflammatory foci and liver steatosis.

SUBMITTER: McManaman JL

PROVIDER: S-EPMC3622329 | biostudies-other | 2013 May

REPOSITORIES: biostudies-other

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Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease.

McManaman James L JL Bales Elise S ES Orlicky David J DJ Jackman Matthew M MacLean Paul S PS Cain Shannon S Crunk Amanda E AE Mansur Ayla A Graham Christine E CE Bowman Thomas A TA Greenberg Andrew S AS

Journal of lipid research 20130212 5

The cytoplasmic lipid droplet (CLD) protein perilipin-2 (Plin2) is expressed in multiple nonadipose tissues, where it is thought to play a role in regulating their lipid storage properties. However, the extent to which Plin2 functions in nutrient utilization and metabolism, or how it influences the consequences of over-feeding, remains unclear. In this study, we demonstrate that the absence of Plin2 prevents high-fat diet(HFD)-induced obesity in male and female mice. This response is associated ...[more]

PMID: 23402988

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Project description:OBJECTIVE:The nuclear receptor TAK1/TR4/NR2C2 is expressed in several tissues that are important in the control of energy homeostasis. In this study, we investigate whether TAK1 functions as a regulator of lipid and energy homeostasis and has a role in metabolic syndrome. RESEARCH DESIGN AND METHODS:We generated TAK1-deficient (TAK1?(/)?) mice to study the function of TAK1 in the development of metabolic syndrome in aged mice and mice fed a high-fat diet (HFD). (Immuno)histochemical, biochemical, and gene expression profile analyses were performed to determine the effect of the loss of TAK1 expression on lipid homeostasis in liver and adipose tissues. In addition, insulin sensitivity, energy expenditure, and adipose-associated inflammation were compared in wild-type (WT) and TAK1?(/)? mice fed a HFD. RESULTS:TAK1-deficient (TAK1?(/)?) mice are resistant to the development of age- and HFD-induced metabolic syndrome. Histo- and biochemical analyses showed significantly lower hepatic triglyceride levels and reduced lipid accumulation in adipose tissue in TAK1?(/)? mice compared with WT mice. Gene expression profiling analysis revealed that the expression of several genes encoding proteins involved in lipid uptake and triglyceride synthesis and storage, including Cidea, Cidec, Mogat1, and CD36, was greatly decreased in the liver and primary hepatocytes of TAK1?(/)? mice. Restoration of TAK1 expression in TAK1?(/)? hepatocytes induced expression of several lipogenic genes. Moreover, TAK1?(/)? mice exhibited reduced infiltration of inflammatory cells and expression of inflammatory genes in white adipose tissue, and were resistant to the development of glucose intolerance and insulin resistance. TAK1?(/)? mice consume more oxygen and produce more carbon dioxide than WT mice, suggesting increased energy expenditure. CONCLUSIONS:Our data reveal that TAK1 plays a critical role in the regulation of energy and lipid homeostasis, and promotes the development of metabolic syndrome. TAK1 may provide a new therapeutic target in the management of obesity, diabetes, and liver steatosis.

Dataset Information

Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease.

Publications

Perilipin-2-null mice are protected against diet-induced obesity, adipose inflammation, and fatty liver disease.

Similar Datasets

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