Leptin Upregulates the Expression of ?3-Integrin, MMP9, HB-EGF, and IL-1? in Primary Porcine Endometrium Epithelial Cells In Vitro.

ABSTRACT: Obesity has become a global health problem. Research suggests that leptin, a hormone that responds to fat deposition, may be involved in mammalian reproduction; however, its precise role in embryo implantation is poorly understood. Here, primary porcine endometrium epithelium cells (PEECs) were cultured in vitro and used to evaluate the regulatory role of different leptin levels on ?3-integrin, MMP9, HB-EGF, and IL-1?, which are, respectively, involved in four critical steps of embryo implantation. Results showed that only 0.01 nM leptin significantly improved ?3-integrin mRNA expression (p < 0.05). MMP9 and HB-EGF mRNA expressions were upregulated by 0.10-10.00 nM leptin (p < 0.05). The IL-1? expression level was only increased by 10.00 nM leptin (p < 0.05). ?3-integrin, MMP9, HB-EGF, and IL-1? mRNA and protein have a similar fluctuant response to increased leptin. Leptin's influence on ?3-integrin, MMP9, HB-EGF, and IL-1? disappeared when the JAK2, PI(3)K, or MAPK signaling pathways were blocked, respectively. In conclusion, leptin affected porcine implantation by regulating the expression of ?3-integrin, MMP9, HB-EGF, and IL-1? in a dose-dependent manner. The signaling pathways of JAK2, PI(3)K, and MAPK may participate in this regulatory process. These findings will contribute to further understanding the mechanisms of reproductive disorders in obesity.

SUBMITTER: Wang H 

PROVIDER: S-EPMC7560211 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature