An Experimentally Defined Hypoxia Gene Signature in Glioblastoma and Its Modulation by Metformin.

ABSTRACT: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor, characterized by a high degree of intertumoral heterogeneity. However, a common feature of the GBM microenvironment is hypoxia, which can promote radio- and chemotherapy resistance, immunosuppression, angiogenesis, and stemness. We experimentally defined common GBM adaptations to physiologically relevant oxygen gradients, and we assessed their modulation by the metabolic drug metformin. We directly exposed human GBM cell lines to hypoxia (1% O₂) and to physioxia (5% O₂). We then performed transcriptional profiling and compared our in vitro findings to predicted hypoxic areas in vivo using in silico analyses. We observed a heterogenous hypoxia response, but also a common gene signature

SUBMITTER: Calvo Tardon M 

PROVIDER: S-EPMC7563149 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature