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Triclosan disrupts immune cell function by depressing Ca²⁺ influx following acidification of the cytoplasm.

ABSTRACT: Triclosan (TCS) is an antimicrobial agent that was effectively banned by the FDA from hand soaps in 2016, hospital soaps in 2017, and hand sanitizers in 2019; however, TCS can still be found in a few products. At consumer-relevant, non-cytotoxic doses, TCS inhibits the functions of both mitochondria and mast cells, a ubiquitous cell type. Via the store-operated Ca²⁺ entry mechanism utilized by many immune cells, mast cells undergo antigen-stimulated Ca²⁺ influx into the cytosol, for proper function. Previous work showed that TCS inhibits Ca²⁺ dynamics in mast cells, and here we show that TCS also inhibits Ca²⁺ mobilization in human Jurkat T cells. However, the biochemical mechanism behind the Ca²⁺ dampening has yet to be elucidated. Three-dimensional super-resolution microscopy reveals that TCS induces mitochondrial swelling, in line with and extending the previous finding of TCS inhibition of mitochondrial membrane potential via its proton ionophoric activity. Inhibition of plasma membrane potential (PMP) by the canonical depolarizer gramicidin can inhibit mast cell function. However, use of the genetically encoded voltage indicators (GEVIs) ArcLight (pH-sensitive) and ASAP2 (pH-insensitive), indicates that TCS does not disrupt PMP. In conjunction with data from a plasma membrane-localized, pH-sensitive reporter, these results indicate that TCS, instead, induces cytosolic acidification in mast cells and T cells. Acidification of the cytosol likely inhibits Ca²⁺ influx by uncoupling the STIM1/ORAI1 interaction that is required for opening of plasma membrane Ca²⁺ channels. These results provide a mechanistic explanation of TCS disruption of Ca²⁺ influx and, thus, of immune cell function.

SUBMITTER: Sangroula S

PROVIDER: S-EPMC7566221 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Triclosan disrupts immune cell function by depressing Ca²⁺ influx following acidification of the cytoplasm.

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Triclosan disrupts immune cell function by depressing Ca2+ influx following acidification of the cytoplasm.

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OmicsDI is part of the ELIXIR infrastructure

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Triclosan disrupts immune cell function by depressing Ca²⁺ influx following acidification of the cytoplasm.