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DIALib-QC an assessment tool for spectral libraries in data-independent acquisition proteomics.

ABSTRACT: Data-independent acquisition (DIA) mass spectrometry, also known as Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH), is a popular label-free proteomics strategy to comprehensively quantify peptides/proteins utilizing mass spectral libraries to decipher inherently multiplexed spectra collected linearly across a mass range. Although there are many spectral libraries produced worldwide, the quality control of these libraries is lacking. We present the DIALib-QC (DIA library quality control) software tool for the systematic evaluation of a library's characteristics, completeness and correctness across 62 parameters of compliance, and further provide the option to improve its quality. We demonstrate its utility in assessing and repairing spectral libraries for correctness, accuracy and sensitivity.

SUBMITTER: Midha MK 

PROVIDER: S-EPMC7567827 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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DIALib-QC an assessment tool for spectral libraries in data-independent acquisition proteomics.

Midha Mukul K MK   Campbell David S DS   Kapil Charu C   Kusebauch Ulrike U   Hoopmann Michael R MR   Bader Samuel L SL   Moritz Robert L RL  

Nature communications 20201016 1

Data-independent acquisition (DIA) mass spectrometry, also known as Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH), is a popular label-free proteomics strategy to comprehensively quantify peptides/proteins utilizing mass spectral libraries to decipher inherently multiplexed spectra collected linearly across a mass range. Although there are many spectral libraries produced worldwide, the quality control of these libraries is lacking. We present the DIALib-QC (DIA library qu  ...[more]