Dataset Information

The DIALib-QC software tool for the systematic assessment of spectral library quality in data-independent acquisition proteomics

ABSTRACT: DIALib-QC (DIA library quality control) tool is used for the systematic evaluation of a spectral library’s characteristics, completeness and mass-accuracy ensuring correct identification and accurate quantitation of peptides and proteins from DIA/SWATH processing tools.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Acute Myeloid Leukemia Cell Line

SUBMITTER: Mukul Midha

LAB HEAD: Prof. Robert L. Moritz

PROVIDER: PXD020953 | Pride | 2020-10-16

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	K562_10ug_DDA_Top100_r01.mzML	Mzml
	K562_10ug_DDA_Top100_r01.wiff	Wiff
	K562_10ug_DDA_Top100_r01.wiff.scan	Wiff
	K562_10ug_DDA_Top100_r02.mzML	Mzml
	K562_10ug_DDA_Top100_r02.wiff	Wiff

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Publications

DIALib-QC an assessment tool for spectral libraries in data-independent acquisition proteomics.

Midha Mukul K MK Campbell David S DS Kapil Charu C Kusebauch Ulrike U Hoopmann Michael R MR Bader Samuel L SL Moritz Robert L RL

Nature communications 20201016 1

Data-independent acquisition (DIA) mass spectrometry, also known as Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH), is a popular label-free proteomics strategy to comprehensively quantify peptides/proteins utilizing mass spectral libraries to decipher inherently multiplexed spectra collected linearly across a mass range. Although there are many spectral libraries produced worldwide, the quality control of these libraries is lacking. We present the DIALib-QC (DIA library qu ...[more]

PMID: 33067471

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