Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC7568196 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Wang Jingge J Huang Chaoyong C Guo Kai K Ma Lianjie L Meng Xiangyu X Wang Ning N Huo Yi-Xin YX
Synthetic and systems biotechnology 20201015 4
<i>Escherichia coli</i> strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655 genome, generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introduc ...[more]