Ontology highlight
ABSTRACT:
SUBMITTER: Heß MC
PROVIDER: S-EPMC7568252 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Heß Marc Christian MC Bloess Svenja S Risse Joe Max JM Friehs Karl K Fischer von Mollard Gabriele G
MicrobiologyOpen 20200827 10
l-amino acid oxidases (LAAOs) are flavoenzymes that catalyze the oxidative deamination of l-amino acids to the corresponding α-keto acids, ammonia, and hydrogen peroxide. Here, we show the overexpression, purification, and the characterization of LAAO4 from the fungus Hebeloma cylindrosporum in the yeast Pichia pastoris with a 9His-tag and compare this with the recently characterized 6His-hcLAAO4 expressed in E. coli. The expression of the enzyme with an ER-signal sequence in P. pastoris resulte ...[more]