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Electron Beam-Induced Transformation in High-Density Amorphous Ices.

ABSTRACT: Amorphous ice is commonly used as a noncrystalline matrix for protecting sensitive biological samples in cryogenic electron microscopy (cryo-EM). The amorphization process of water is complex, and at least two amorphous states of different densities are known to exist, high- and low-density amorphous ices (HDA and LDA). These forms are considered to be the counterparts of two distinct liquid states, namely, high- and low-density liquid water. Herein, we investigate the HDA to LDA transition using electron diffraction and cryo-EM. The observed phase transition is induced by the impact of electrons, and we discuss two different mechanisms, namely, local heating and beam-induced motion of water molecules. The temperature increase is estimated by comparison with X-ray scattering experiments on identically prepared samples. Our results suggest that HDA, under the conditions used in our cryo-EM measurements, is locally heated above its glass-transition temperature.

SUBMITTER: Xu H

PROVIDER: S-EPMC7569672 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Electron Beam-Induced Transformation in High-Density Amorphous Ices.

Xu Hongyi H Ångström Jonas J Eklund Tobias T Amann-Winkel Katrin K

The journal of physical chemistry. B 20200930 41

Amorphous ice is commonly used as a noncrystalline matrix for protecting sensitive biological samples in cryogenic electron microscopy (cryo-EM). The amorphization process of water is complex, and at least two amorphous states of different densities are known to exist, high- and low-density amorphous ices (HDA and LDA). These forms are considered to be the counterparts of two distinct liquid states, namely, high- and low-density liquid water. Herein, we investigate the HDA to LDA transition usin ...[more]

PMID: 32997503

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