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Conformation of G-quadruplex Controlled by Click Reaction.

ABSTRACT: G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2'-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence.

SUBMITTER: Xiao CD

PROVIDER: S-EPMC7570587 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Conformation of G-quadruplex Controlled by Click Reaction.

Xiao Chao-Da CD He Zhi-Yong ZY Guo Chuan-Xin CX Shen Xiang-Chun XC Xu Yan Y

Molecules (Basel, Switzerland) 20200922 18

G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2'-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel ...[more]

PMID: 32971833

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