Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such as Synechocystis sp. PCC 6803.
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ABSTRACT:
SUBMITTER: Caicedo-Burbano P
PROVIDER: S-EPMC7573980 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
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Construction of Fully Segregated Genomic Libraries in Polyploid Organisms Such as <i>Synechocystis</i> sp. PCC 6803.
Caicedo-Burbano Patricia P Smit Tycho T Pineda Hernández Hugo H Du Wei W Branco Dos Santos Filipe F
ACS synthetic biology 20201005 10
Several microbes are polyploid, meaning they contain several copies of their chromosome. Cyanobacteria, while holding great potential as photosynthetic cell factories of various products, are found among them. In these clades the diversity of genetic elements that serve within the basic molecular toolbox is often limiting. To assist mining for the latter, we present here a method for the generation of fully segregated genomic libraries, specifically designed for polyploids. We provide proof-of-p ...[more]
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