Dataset Information

Unbiased Screens Show CD8⁺ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

ABSTRACT: Developing effective strategies to prevent or treat coronavirus disease 2019 (COVID-19) requires understanding the natural immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We used an unbiased, genome-wide screening technology to determine the precise peptide sequences in SARS-CoV-2 that are recognized by the memory CD8⁺ T cells of COVID-19 patients. In total, we identified 3-8 epitopes for each of the 6 most prevalent human leukocyte antigen (HLA) types. These epitopes were broadly shared across patients and located in regions of the virus that are not subject to mutational variation. Notably, only 3 of the 29 shared epitopes were located in the spike protein, whereas most epitopes were located in ORF1ab or the nucleocapsid protein. We also found that CD8⁺ T cells generally do not cross-react with epitopes in the four seasonal coronaviruses that cause the common cold. Overall, these findings can inform development of next-generation vaccines that better recapitulate natural CD8⁺ T cell immunity to SARS-CoV-2.

SUBMITTER: Ferretti AP

PROVIDER: S-EPMC7574860 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Unbiased Screens Show CD8<sup>+</sup> T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

Ferretti Andrew P AP Kula Tomasz T Wang Yifan Y Nguyen Dalena M V DMV Weinheimer Adam A Dunlap Garrett S GS Xu Qikai Q Nabilsi Nancy N Perullo Candace R CR Cristofaro Alexander W AW Whitton Holly J HJ Virbasius Amy A Olivier Kenneth J KJ Buckner Lyndsey R LR Alistar Angela T AT Whitman Eric D ED Bertino Sarah A SA Chattopadhyay Shrikanta S MacBeath Gavin G

Immunity 20201020 5

Developing effective strategies to prevent or treat coronavirus disease 2019 (COVID-19) requires understanding the natural immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We used an unbiased, genome-wide screening technology to determine the precise peptide sequences in SARS-CoV-2 that are recognized by the memory CD8<sup>+</sup> T cells of COVID-19 patients. In total, we identified 3-8 epitopes for each of the 6 most prevalent human leukocyte antigen (HLA) types ...[more]

PMID: 33128877

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Dataset Information

Unbiased Screens Show CD8⁺ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

Publications

Unbiased Screens Show CD8<sup>+</sup> T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

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Dataset Information

Unbiased Screens Show CD8+ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

Publications

Unbiased Screens Show CD8<sup>+</sup> T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Unbiased Screens Show CD8⁺ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein.