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Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research.

ABSTRACT: The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dietary impact on cecal microbiota, short- and branched-chain fatty acid profiles was evaluated. The gut microbiota composition differed significantly between diets and sexes, with significantly different clusters in ?-diversity. Total BCFA were highest (p?=?0.01) and SCFA were lowest (p?=?0.03) in mice fed a diet lower in FODMAPs and gluten. These results suggest that nutritional composition of commercially available rodent diets impact gut microbiota profiles and fermentation patterns, with major implications for the reproducibility of results across laboratories. However, further studies are required to elucidate the specific dietary factors driving these changes.

SUBMITTER: Tuck CJ

PROVIDER: S-EPMC7575541 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research.

Tuck C J CJ De Palma G G Takami K K Brant B B Caminero A A Reed D E DE Muir J G JG Gibson P R PR Winterborn A A Verdu E F EF Bercik P P Vanner S S

Scientific reports 20201020 1

The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, ...[more]

PMID: 33082369

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Project description:The cardiovascular and other health benefits and potential harms of protein and micronutrient deficiency of vegetarian diets continue to be debated.Study participants included urban migrants, their rural siblings and urban residents (n = 6555, mean age - 40.9 yrs) of the Indian Migration Study from Lucknow, Nagpur, Hyderabad and Bangalore. Information on diet (validated interviewer-administered semi-quantitative food frequency questionnaire), tobacco, alcohol, physical activity, medical histories, as well as blood pressure, fasting blood and anthropometric measurements were collected. Nutrient databases were used to calculate nutrient content of regional recipes. Vegetarians ate no eggs, fish, poultry and meat. Using multivariate linear regression with robust standard error model, we compared the macro- and micro-nutrient profile of vegetarian and non-vegetarian diets.Vegetarians, (32.8% of the population), consumed greater amounts of legumes, vegetables, roots and tubers, dairy and sugar, while non-vegetarians had a greater intake of cereals, fruits, spices, salt (p?<?0.01), fats and oils. Vegetarians had a higher socioeconomic status, and were less likely to smoke, drink alcohol (p?<?0.0001) and engage in less physical activity (p?=?0.04). On multivariate analysis, vegetarians consumed more carbohydrates (??=?7.0 g/day (95% CI: 9.9 to 4.0), p?<?0.0001), vitamin C (??=?8.7 mg/day (95% CI: 4.3 to13.0), p?<?0.0001) and folate (??=?8.0 mcg/day (95% CI: 3.3 to 12.7), p?=?0.001) and lower levels of fat (??=?-1.6 g/day (95% CI: -0.62 to -2.7), p?=?0.002), protein (??=?-6.4 g/day (95% CI: -5.8 to -7.0), p?<?0.0001), vitamin B12 (??=?-1.4 mcg/day (95% CI: -1.2 to -1.5), p?<?0.0001) and zinc (??=?-0.6 mg/day (95% CI: -0.4 to -0.7), p?<?0.0001).Overall, Indian vegetarian diets were found to be adequate to sustain nutritional demands according to recommended dietary allowances with less fat. Lower vitamin B12 bio-availability remains a concern and requires exploration of acceptable dietary sources for vegetarians.

Dataset Information

Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research.

Publications

Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research.

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