Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe M
PROVIDER: S-EPMC7576197 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Watanabe Masashi M Saeki Yasushi Y Takahashi Hidehisa H Ohtake Fumiaki F Yoshida Yukiko Y Kasuga Yusuke Y Kondo Takeshi T Yaguchi Hiroaki H Suzuki Masanobu M Ishida Hiroki H Tanaka Keiji K Hatakeyama Shigetsugu S
Communications biology 20201020 1
The identification of true substrates of an E3 ligase is biologically important but biochemically difficult. In recent years, several techniques for identifying substrates have been developed, but these approaches cannot exclude indirect ubiquitination or have other limitations. Here we develop an E3 ligase substrate-trapping strategy by fusing a tandem ubiquitin-binding entity (TUBE) with an anti-ubiquitin remnant antibody to effectively identify ubiquitinated substrates. We apply this method t ...[more]