The relationship between the commensal microbiota levels and Crohn's disease activity.

ABSTRACT: Background and aim:Human gut microbiota play an important role in metabolism and host physiology. Perturbations of the gut microbial communities lead to the development of various diseases such as inflammatory bowel disease, celiac disease, allergic diseases, and metabolic diseases. Crohn's disease is a chronic inflammatory bowel disease characterized by periods of remission and relapse. Several studies suggest that intestinal inflammation arises due to an abnormal response of the intestinal immune system to the fecal microbiota. The goal of the study was to evaluate the relative amount of four bacterial groups in fecal samples of Crohn's disease patients and their relation to the inflammatory activity. Methods:We studied stool samples of 105 individuals, 54 with Crohn's disease and 51 as a control group. The DNA extracted from the stool samples was subjected to real-time polymerase chain reaction (qPCR) for quantification of the Bacteroidetes phylum, class Bacilli, and Bifidobacteriaceae and Enterobacteriaceae families. Results:We found a significant increase in Bacteroidetes in Crohn's disease samples when compared to the control group (14?650 and 2060?CFU/ng DNA, respectively) (P = 0.014). On the other hand, we observed a significant reduction in Bacilli and Bifidobacteriaceae (13 and 58?CFU/ng DNA, respectively) (P?

SUBMITTER: de Alencar Junior H 

PROVIDER: S-EPMC7578322 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature