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Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.

ABSTRACT: The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.

SUBMITTER: Kleinsorge M

PROVIDER: S-EPMC7580117 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.

Kleinsorge Mandy M Cyganek Lukas L

STAR protocols 20200603 1

The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018. ...[more]

PMID: 33111079

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