Ontology highlight
ABSTRACT:
SUBMITTER: Lissandrello CA
PROVIDER: S-EPMC7582186 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Lissandrello Charles A CA Santos Jose A JA Hsi Peter P Welch Michaela M Mott Vienna L VL Kim Ernest S ES Chesin Jordan J Haroutunian Nerses J NJ Stoddard Aaron G AG Czarnecki Andrew A Coppeta Jonathan R JR Freeman Daniel K DK Flusberg Deborah A DA Balestrini Jenna L JL Tandon Vishal V
Scientific reports 20201022 1
Implementation of gene editing technologies such as CRISPR/Cas9 in the manufacture of novel cell-based therapeutics has the potential to enable highly-targeted, stable, and persistent genome modifications without the use of viral vectors. Electroporation has emerged as a preferred method for delivering gene-editing machinery to target cells, but a major challenge remaining is that most commercial electroporation machines are built for research and process development rather than for large-scale, ...[more]