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Compromised browning plasticity of primary subcutaneous adipocytes derived from overweight Chinese adults.

ABSTRACT: Purpose:People with obesity have a compromised browning capacity of adipose tissue when faced with sympathetic stimuli. This study aimed to determine whether norepinephrine treatment can enhance the induction of precursor cells from human white adipose tissue to differentiate into adipocytes that express key markers of beige adipocytes, and if there is a difference in this capacity between normal weight and overweight individuals. Methods:Stromal vascular cells derived from subcutaneous white adipose tissue of normal weight and overweight groups were induced to differentiation, with or without norepinephrine, into adipocytes. Oxygen consumption rate, lipolysis, the expression of uncoupling protein 1 and other thermogenic genes were compared between different adiposity and treatment groups. Results:Peroxisome proliferator activated receptor ?- coactivator-1 alpha (PGC-1 ?) and uncoupling protein 1 gene expression increased significantly in the normal weight group, but not in the overweight group, with norepinephrine treatment. The increments of lipolysis and oxygen consumption rate were also higher in adipocytes from the normal weight group with norepinephrine treatment, as compared with those of the overweight group. PR domain containing protein 16 (PRDM 16) gene expression was higher in the normal weight group compared with that in the overweight group, while there were no significant changes found with norepinephrine treatment in either the normal weight or overweight group. Conclusions:Adipogenic precursor cells derived from overweight individuals were less prone to differentiate into beige-like adipocytes when facing sympathetic stimuli than normal weight ones, resulting in the compromised sympathetic-induced browning capacity in subcutaneous white adipose tissue in overweight individuals, which occurred before the onset of overt obesity.

SUBMITTER: Qiu Y

PROVIDER: S-EPMC7583200 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Compromised browning plasticity of primary subcutaneous adipocytes derived from overweight Chinese adults.

Qiu Yao Y Sun Lizhi L Hu Xiaolin X Zhao Xin X Shi Hongyan H Liu Zhao Z Yin Xiao X

Diabetology & metabolic syndrome 20201022

<h4>Purpose</h4>People with obesity have a compromised browning capacity of adipose tissue when faced with sympathetic stimuli. This study aimed to determine whether norepinephrine treatment can enhance the induction of precursor cells from human white adipose tissue to differentiate into adipocytes that express key markers of beige adipocytes, and if there is a difference in this capacity between normal weight and overweight individuals.<h4>Methods</h4>Stromal vascular cells derived from subcut ...[more]

PMID: 33110450

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Project description:Recent US work identified "metabolically healthy overweight" and "metabolically at risk normal weight" individuals. Less is known for modernizing countries with recent increased obesity.Fasting blood samples, anthropometry and blood pressure from 8,233 adults aged 18-98 in the 2009 nationwide China Health and Nutrition Survey, were used to determine prevalence of overweight (Asian cut point, BMI ? 23 kg/m(2) ) and five risk factors (prediabetes/diabetes (hemoglobin A1c ? 5.7%) inflammation (high-sensitivity C-reactive protein (hsCRP) ? 3 mg/l), prehypertension/hypertension (Systolic blood pressure/diastolic blood pressure ? 130/85 mm Hg), high triglycerides (? 150 mg/dl), low high-density lipoprotein cholesterol (<40 (men)/ <50 mg/dl (women)). Sex-stratified, logistic, and multinomial logistic regression models estimated concurrent obesity and cardiometabolic risk, with and without abdominal obesity, adjusting for age, smoking, alcohol consumption, physical activity, urbanicity, and income.Irrespective of urbanicity, 78.3% of the sample had ? 1 elevated cardiometabolic risk factor (normal weight: 33.2% had ? 1 elevated risk factor; overweight: 5.7% had none). At the age of 18-30 years, 47.4% had no elevated risk factors, which dropped to 6% by the age 70, largely due to age-related increase in hypertension risk (18-30 years: 11%; >70 years: 73%). Abdominal obesity was highly predictive of metabolic risk, irrespective of overweight (e.g., "metabolically at risk overweight" relative to "metabolically healthy normal weight" (men: relative risk ratio (RRR) = 39.06; 95% confidence interval (CI): 23.47, 65.00; women: RRR = 22.26; 95% CI: 17.49, 28.33)).A large proportion of Chinese adults have metabolic abnormalities. High hypertension risk with age, underlies the low prevalence of metabolically healthy overweight. Screening for cardiometabolic-related outcomes dependent upon overweight will likely miss a large portion of the Chinese at risk population.

Dataset Information

Compromised browning plasticity of primary subcutaneous adipocytes derived from overweight Chinese adults.

Publications

Compromised browning plasticity of primary subcutaneous adipocytes derived from overweight Chinese adults.

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