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Co-Occurrence of the mcr-1.1 and mcr-3.7 Genes in a Multidrug-Resistant Escherichia coli Isolate from China.

ABSTRACT: Objective:A colistin-resistant Escherichia coli strain isolated from dog feces was characterized in this study. Methods and Results:A multiplex PCR assay was used to detect the presence of colistin-resistant mcr genes; it was found that E. coli QDFD216 co-harbored the mcr-1 and mcr-3 genes. Whole-genome sequencing and further bioinformatics analysis revealed that E. coli QDFD216 belonged to serotype O176:H11, fimH1311 type and ST132. The resistance genes bla CTX-M-14, mdfA, dfrA3, acrA, acrB, tolc, and sul3 were present in the chromosome. The mcr-1.1 and mcr-3.7 genes were located in two plasmids of different incompatibility groups. mcr-1.1 was carried by a IncX4-type plasmid within an typical IS26-parA-mcr-1.1-pap2 cassette, while mcr-3.7 was encoded by an IncP1-type plasmid with a genetic structure of TnAs2-mcr-3.7-dgkA-IS26. No additional antibiotic resistance genes were carried by either plasmid. Conclusion:This is the first report of an E. coli isolate co-harboring a mcr-1.1-carrying IncX4 plasmid and a mcr-3.7-carrying IncP1 plasmid. The evolution and mechanism of mcr gene co-existence need further study to assess its impact on public health.

SUBMITTER: Du C 

PROVIDER: S-EPMC7585518 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Co-Occurrence of the <i>mcr-1.1</i> and <i>mcr-3.7</i> Genes in a Multidrug-Resistant <i>Escherichia coli</i> Isolate from China.

Du Chongtao C   Feng Yuyang Y   Wang Guizhen G   Zhang Zhiyuan Z   Hu Huimin H   Yu Yu Y   Liu Jiayang J   Qiu Lihao L   Liu Hongtao H   Guo Zhimin Z   Huang Jing J   Qiu Jiazhang J  

Infection and drug resistance 20201019

<h4>Objective</h4>A colistin-resistant <i>Escherichia coli</i> strain isolated from dog feces was characterized in this study.<h4>Methods and results</h4>A multiplex PCR assay was used to detect the presence of colistin-resistant <i>mcr</i> genes; it was found that <i>E. coli</i> QDFD216 co-harbored the <i>mcr-1</i> and <i>mcr-3</i> genes. Whole-genome sequencing and further bioinformatics analysis revealed that <i>E. coli</i> QDFD216 belonged to serotype O176:H11, fimH1311 type and ST132. The r  ...[more]

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