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Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani.

ABSTRACT: Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

SUBMITTER: Pedersen TB 

PROVIDER: S-EPMC7589453 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Through stepwise recreation of the biosynthetic gene cluster containing <i>PKS3</i> from <i>Fusarium solani</i>, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in <i>Saccharomyces cerevisiae</i>. This was achieved through sequential transformation associated recombination (TAR) cloning of <i>FvPPT</i>, <i>fsr1</i>, <i>fsr2</i>, and <i>fsr3</i> into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterolo  ...[more]

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