Dataset Information

NGF/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells.

ABSTRACT: Nerve Growth Factor (NGF) and its high-affinity receptor tropomyosin receptor kinase A (TRKA) increase their expression during the progression of epithelial ovarian cancer (EOC), promoting cell proliferation and angiogenesis through several oncogenic proteins, such as c-MYC and vascular endothelial growth factor (VEGF). The expression of these proteins is controlled by microRNAs (miRs), such as miR-145, whose dysregulation has been related to cancer. The aims of this work were to evaluate in EOC cells whether NGF/TRKA decreases miR-145 levels, and the effect of miR-145 upregulation. The levels of miR-145-5p were assessed by qPCR in ovarian biopsies and ovarian cell lines (human ovarian surface epithelial cells (HOSE), A2780 and SKOV3) stimulated with NGF. Overexpression of miR-145 in ovarian cells was used to evaluate cell proliferation, migration, invasion, c-MYC and VEGF protein levels, as well as tumor formation and metastasis in vivo. In EOC samples, miR-145-5p levels were lower than in epithelial ovarian tumors. Overexpression of miR-145 decreased cell proliferation, migration and invasion of EOC cells, changes that were concomitant with the decrease in c-MYC and VEGF protein levels. We observed decreased tumor formation and suppressed metastasis behavior in mice injected with EOC cells that overexpressed miR-145. As expected, ovarian cell lines stimulated with NGF diminished miR-145-5p transcription and abundance. These results suggest that the tumoral effects of NGF/TRKA depend on the regulation of miR-145-5p levels in EOC cells, and that its upregulation could be used as a possible therapeutic strategy for EOC.

SUBMITTER: Garrido MP

PROVIDER: S-EPMC7589588 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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NGF/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells.

Garrido Maritza P MP Torres Ignacio I Avila Alba A Chnaiderman Jonás J Valenzuela-Valderrama Manuel M Aramburo José J Oróstica Lorena L Durán-Jara Eduardo E Lobos-Gonzalez Lorena L Romero Carmen C

International journal of molecular sciences 20201016 20

Nerve Growth Factor (NGF) and its high-affinity receptor tropomyosin receptor kinase A (TRKA) increase their expression during the progression of epithelial ovarian cancer (EOC), promoting cell proliferation and angiogenesis through several oncogenic proteins, such as c-MYC and vascular endothelial growth factor (VEGF). The expression of these proteins is controlled by microRNAs (miRs), such as miR-145, whose dysregulation has been related to cancer. The aims of this work were to evaluate in EOC ...[more]

PMID: 33081171

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Dataset Information

NGF/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells.

Publications

NGF/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells.

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