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Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types.

ABSTRACT: Neural circuit analysis relies on having molecular markers for specific cell types. However, for a cell type identified only by its circuit function, the process of identifying markers remains laborious. We developed physiological optical tagging sequencing (PhOTseq), a technique for tagging and expression profiling of cells on the basis of their functional properties. PhOTseq was capable of selecting rare cell types and enriching them by nearly 100-fold. We applied PhOTseq to the challenge of mapping receptor-ligand pairings among pheromone-sensing neurons in mice. Together with in vivo ectopic expression of vomeronasal chemoreceptors, PhOTseq identified the complete combinatorial receptor code for a specific set of ligands.

SUBMITTER: Lee D

PROVIDER: S-EPMC7591936 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types.

Lee Donghoon D Kume Maiko M Holy Timothy E TE

Science (New York, N.Y.) 20191201 6471

Neural circuit analysis relies on having molecular markers for specific cell types. However, for a cell type identified only by its circuit function, the process of identifying markers remains laborious. We developed physiological optical tagging sequencing (PhOTseq), a technique for tagging and expression profiling of cells on the basis of their functional properties. PhOTseq was capable of selecting rare cell types and enriching them by nearly 100-fold. We applied PhOTseq to the challenge of m ...[more]

PMID: 31831669

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