A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene.
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ABSTRACT:
SUBMITTER: Wen J
PROVIDER: S-EPMC7597837 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Publications
A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene.
Wen Junping J Gou Hongchao H Zhan Zeqiang Z Gao Yuan Y Chen Zhengquan Z Bai Jie J Wang Shaojun S Chen Kaifeng K Lin Qijie Q Liao Ming M Zhang Jianmin J
Poultry science 20200425 7
Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.3 × 10<sup>1</sup> CFU/mL, and the method was highly specific and showed a high cli ...[more]
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