Dataset Information

Growth performance and amino acid digestibility responses of broiler chickens fed diets containing purified soybean trypsin inhibitor and supplemented with a monocomponent protease.

ABSTRACT: Trypsin inhibitors (TI) resident in soybeans affects protein utilization. While heat treatment influences residual TI, it simultaneously affects the structure and solubility of the soybean proteins and confounds any response to exogenous proteases. Using purified TI, the effect of exogenous protease to TI can be dissociated from changes in the soybean protein. Thus, the current study was designed to evaluate the growth performance and protein utilization responses of broiler chickens to purified TI and exogenous protease. Soybean meal (SBM) was preanalyzed for basal TI (2,996 TIU/g SBM), formulated into nutritionally adequate experimental diets to contain 1,033 TIU/g diet, and purified TI was added at 9,000 TIU/g diet. A total of 320 Cobb-500 broiler chicks were allocated to 4 diets, each with 8 replicate cages and 10 birds per replicate. The experimental diets were arranged as a 2 × 2 factorial with factors being dietary TI (1,033 or 10,033 TIU/g) and exogenous protease (0 or 15,000 PROT/kg). On day 7, 14, and 21 posthatching, protease supplementation improved the BW gain (P < 0.01) and gain to feed ratio (P < 0.05) of birds. On day 14 and 21 posthatching, the relative weight of pancreas increased (P < 0.05) with added TI but was reduced (P < 0.001) with protease supplementation. Apparent ileal digestibility of all amino acids, except methionine, decreased (P < 0.001) with added TI but increased (P < 0.05) with protease supplementation. Jejunal MUC-2 was downregulated (P < 0.01) and SCL7A-2 was upregulated (P < 0.05) by protease supplementation. Duodenal trypsin and chymotrypsin activities reduced (P < 0.05) with added TI but increased (P < 0.01) with protease supplementation. Exogenous protease produced longer villi (P < 0.05) and deeper crypts (P < 0.01) in the jejunal tissue. In conclusion, dietary addition of purified TI negatively affects nutrient utilization by broiler chickens. Furthermore, the study showed that the efficacy of the exogenous protease might be independent of dietary TI concentration.

SUBMITTER: Aderibigbe A

PROVIDER: S-EPMC7598323 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Growth performance and amino acid digestibility responses of broiler chickens fed diets containing purified soybean trypsin inhibitor and supplemented with a monocomponent protease.

Aderibigbe A A Cowieson A J AJ Sorbara J O JO Pappenberger G G Adeola O O

Poultry science 20200708 10

Trypsin inhibitors (TI) resident in soybeans affects protein utilization. While heat treatment influences residual TI, it simultaneously affects the structure and solubility of the soybean proteins and confounds any response to exogenous proteases. Using purified TI, the effect of exogenous protease to TI can be dissociated from changes in the soybean protein. Thus, the current study was designed to evaluate the growth performance and protein utilization responses of broiler chickens to purified ...[more]

PMID: 32988537

Similar Datasets

Project description:The objective of this study was to determine the effects of protease origin and dosage on the prececal (pc) amino acid (AA) digestibility and the influence on composition of the microbial community in the small intestine. In addition, the effects of phytase supplementation were investigated. A total of 8 dietary treatments were included. The basal diet contained mainly corn and soybean meal. Three protease products were added to the basal diet, each at the level recommended by the supplier and at an 8-fold level. Phytase was supplemented in another dietary treatment. Each dietary treatment was allocated to 8 replicates of 15 birds each. The experimental diets were offered from day 15 to 21 for ad libitum consumption. The effect of protease supplementation on the pc AA digestibility depended on the protease product type and the amount supplemented. The pc AA digestibility was significantly increased by 1 protease product when supplemented at high level and when phytase was supplemented. In all the other treatments, protease supplementation had no significant influence or it decreased pc AA digestibility, when compared with the treatment with no enzymes added. In general, Firmicutes was the most abundant phylum among the ileal microbiota across all the treatments. Significant effects on microbiota composition were observed at the genus level for some but not all protease treatments and phytase supplementation. The genera Streptococcus, Lactobacillus, and uncultured Clostridiaceae were responsible for these differences. Furthermore, microbial networks established for each diet showed either high or low number of intergeneric interactions, but without a consistent enzyme effect. We conclude that enzyme supplementation effects were evident in the terminal small intestine microbiota composition, and to a lesser extent, in pc AA digestibility. However, the changes in microbiota composition and pc AA digestibility could not be correlated, indicating absence of a causal relationship.

Project description:The effects of inclusion levels of a phytogenic feed additive (PFA), characterized by menthol anethol and eugenol, on broiler growth performance, nutrient digestibility, biochemical biomarkers and total antioxidant capacity (TAC) of plasma and meat, as well as on the relative expression of selected cytokines, were studied in a 42-d experiment. A total of 225 one-day-old male Cobb broiler chickens were assigned into 3 treatments, with 5 replicates of 15 chickens each. Chickens were fed maize-soybean meal basal diets following a 3 phase (i.e., starter, grower and finisher) feeding program. Depending on PFA inclusion level, treatments were: no PFA (PFA-0), PFA at 100 mg/kg (PFA-100) and PFA at 150 mg/kg (PFA-150). Feed and water were available ad libitum. Feed conversion ratio (FCR) during finisher phase was improved quadratically (P < 0.05) with increasing PFA level. Overall, increasing PFA level increased body weight gain (BWG) in a linear (P < 0.05) and quadratic (P < 0.05) manner with treatments PFA-100 and PFA-150 being greater (P < 0.05) compared with PFA-0. Total tract apparent digestibility of dry matter increased linearly (P < 0.05) and quadratically (P < 0.05) with increasing PFA level. The apparent metabolizable energy corrected for nitrogen (AMEn) also increased linearly (P < 0.05). Increasing PFA level resulted in a linear (P < 0.05) increase in blood plasma TAC. Expression of pro-inflammatory cytokine interleukin -18 (IL-18) was reduced linearly (P < 0.05) in spleen with increasing PFA level. In conclusion, PFA inclusion at 100 mg/kg diet positively influenced performance, whereas PFA inclusion at 150 mg/kg resulted in a stronger improvement in AMEn and plasma TAC. Finally, PFA inclusion resulted in a pattern of reduced pro-inflammatory biomarker IL-18 at spleen. Overall, this study provides evidence for the beneficial role of PFA as a natural growth and health promoter in broiler chickens that needs to be further confirmed in field studies.

Project description:The aim of this work was to study the continuous or intermittent impact of a multienzyme supplement on growth performance, nutrient digestibility, and blood metabolites of broilers, and to evaluate production index of dietary supplementation. A total of 315 unsexed day-old Arbor Acres broiler chicks were randomly distributed to seven treatments groups, keeping initial body weights similar, in 35-floor pens (replicates) of nine chicks per replicate (pen) and five experimental units per treatment. All experimental groups were fed the same basal diet and administered seven multienzyme treatments: the 1st group (control) did not receive any enzyme supplementation; the 2nd, 3rd, and 4th groups were administered multienzymes at 0.5, 1.0, and 1.5 mL/L drinking water, respectively. Each enzyme supplemented-group was divided into two subgroups, with additives being applied either continuously (24 h/day) or intermittently (12 h/day) from 1 to 35 days old. Regardless of administration method, multienzyme supplements at 1.0 mL/L water along with a corn-soybean meal diet increased the body weight gain (BWG) by 7.8% compared to 0.5 mL/L water during days 1-21 of age. In addition, 1.5 mL/L water significantly improved BWG by 5.1% of broilers compared to 0.5 mL/L water during days 1-35 of age. Enzyme supplementation at 1.5 mL/L water significantly enhanced feed conversion ratio (FCR) by 4.3% during days 1-21 of age, and FCR by 5.2% and European production index (EPI) by 10.4% during days 1-35 of age compared to the group on 0.5 mL/L water. For the whole period, there were improvements of beneficial consideration in BWG (4.0%), FCR (4.0%), and European production index (8.2%) due to continuous multienzyme supplementation at 0.5 mL/L water compared to the same dose added intermittently. A similar trend was observed due to intermittent multienzymes at 1 mL/L drinking water that resulted in increased BWG by (6.4%) and improved FCR by (6.7%) and EPI by (12.7%). Intermittent administration significantly increased feed intake of broilers during 22-35 days of age compared to continuous supplementation. Multienzymes at different doses did not significantly affect the digestibility of nutrients, blood serum biochemical constituent, inner body organs, and markers of functions of liver and renal organs. In conclusion, the highest BWG and the best FCR and EPI for the whole period were from broilers given continuous 1 and 1.5 mL/L drinking water or intermittent multienzyme supplementation at 1.5 mL/L drinking water. Furthermore, intermittent supplementation of enzymes at 1 mL/L drinking water and continuous supplementation at 0.5 mL/L drinking water can be investigated in further experiments as a tool to improve broiler growth performance and economic traits and to decrease the cost of enzyme application.

Dataset Information

Growth performance and amino acid digestibility responses of broiler chickens fed diets containing purified soybean trypsin inhibitor and supplemented with a monocomponent protease.

Publications

Growth performance and amino acid digestibility responses of broiler chickens fed diets containing purified soybean trypsin inhibitor and supplemented with a monocomponent protease.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets