Ontology highlight
ABSTRACT:
SUBMITTER: Ruhlandt D
PROVIDER: S-EPMC7599333 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Ruhlandt Daja D Andresen Martin M Jensen Nickels N Gregor Ingo I Jakobs Stefan S Enderlein Jörg J Chizhik Alexey I AI
Communications biology 20201030 1
One of the key photophysical properties of fluorescent proteins that is most difficult to measure is the quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it is unknown how many of the proposedly fluorescent molecules of a sample are indeed fluorescent (for example due to incomplete maturation, or the presence of photophysical dark states). Here, we use a plasmonic n ...[more]