Unknown

Dataset Information

0

Type I Interferon Transcriptional Network Regulates Expression of Coinhibitory Receptors in Human T cells.


ABSTRACT: While inhibition of T cell co-inhibitory receptors has revolutionized cancer therapy, the mechanisms governing their expression on human T cells have not been elucidated. Type 1 interferon (IFN-I) modulates T cell immunity in viral infection, autoimmunity, and cancer, and may facilitate induction of T cell exhaustion in chronic viral infection 1,2 . Here we show that IFN-I regulates co-inhibitory receptors expression on human T cells, inducing PD-1/TIM-3/LAG-3 while surprisingly inhibiting TIGIT expression. High-temporal-resolution mRNA profiling of IFN-I responses enabled the construction of dynamic transcriptional regulatory networks uncovering three temporal transcriptional waves. Perturbation of key transcription factors on human primary T cells revealed both canonical and non-canonical IFN-I transcriptional regulators, and identified unique regulators that control expression of co-inhibitory receptors. To provide direct in vivo evidence for the role of IFN-I on co-inhibitory receptors, we then performed single cell RNA-sequencing in subjects infected with SARS-CoV-2, where viral load was strongly associated with T cell IFN-I signatures. We found that the dynamic IFN-I response in vitro closely mirrored T cell features with acute IFN-I linked viral infection, with high LAG3 and decreased TIGIT expression. Finally, our gene regulatory network identified SP140 as a key regulator for differential LAG3 and TIGIT expression. The construction of co-inhibitory regulatory networks induced by IFN-I with identification of unique transcription factors controlling their expression may provide targets for enhancement of immunotherapy in cancer, infectious diseases, and autoimmunity.

SUBMITTER: Sumida TS 

PROVIDER: S-EPMC7605554 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Type I Interferon Transcriptional Network Regulates Expression of Coinhibitory Receptors in Human T cells.

Sumida Tomokazu S TS   Dulberg Shai S   Schupp Jonas J   Stillwell Helen A HA   Axisa Pierre-Paul PP   Comi Michela M   Lincoln Matthew M   Unterman Avraham A   Kaminski Naftali N   Madi Asaf A   Kuchroo Vijay K VK   Hafler David A DA  

bioRxiv : the preprint server for biology 20201031


While inhibition of T cell co-inhibitory receptors has revolutionized cancer therapy, the mechanisms governing their expression on human T cells have not been elucidated. Type 1 interferon (IFN-I) modulates T cell immunity in viral infection, autoimmunity, and cancer, and may facilitate induction of T cell exhaustion in chronic viral infection <sup>1,2</sup> . Here we show that IFN-I regulates co-inhibitory receptors expression on human T cells, inducing PD-1/TIM-3/LAG-3 while surprisingly inhib  ...[more]

Similar Datasets

| S-EPMC8989655 | biostudies-literature
| S-EPMC8202434 | biostudies-literature
2022-01-31 | GSE195543 | GEO
2022-01-31 | GSE195540 | GEO
2022-01-31 | GSE195542 | GEO
2022-01-31 | GSE195541 | GEO
| PRJNA801234 | ENA
| PRJNA801421 | ENA
| PRJNA801420 | ENA
| PRJNA801235 | ENA