An optimized acetylcholine sensor for monitoring in vivo cholinergic activity.
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ABSTRACT:
SUBMITTER: Jing M
PROVIDER: S-EPMC7606762 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Publications
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity.
Jing Miao M Li Yuexuan Y Zeng Jianzhi J Huang Pengcheng P Skirzewski Miguel M Kljakic Ornela O Peng Wanling W Qian Tongrui T Tan Ke K Zou Jing J Trinh Simon S Wu Runlong R Zhang Shichen S Pan Sunlei S Hires Samuel A SA Xu Min M Li Haohong H Saksida Lisa M LM Prado Vania F VF Bussey Timothy J TJ Prado Marco A M MAM Chen Liangyi L Cheng Heping H Li Yulong Y
Nature methods 20200928 11
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRAB<sub>ACh</sub> (GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways. The improved version of the ACh sensor retains the subsecond response kinetics, physiologically relevant affinity and precise molecular specificity for ACh of its p ...[more]
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