Unknown

Dataset Information

0

Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase.


ABSTRACT: We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme-probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.

SUBMITTER: George JT 

PROVIDER: S-EPMC7611084 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3045586 | biostudies-literature
| S-EPMC4342108 | biostudies-literature
| S-EPMC5240791 | biostudies-literature
| S-EPMC4317284 | biostudies-literature
| S-EPMC7611130 | biostudies-literature
| S-EPMC6344859 | biostudies-literature
| S-EPMC1524887 | biostudies-literature
| S-EPMC3711335 | biostudies-literature
| S-EPMC4357723 | biostudies-literature
| S-EPMC8608314 | biostudies-literature