Ontology highlight
ABSTRACT:
SUBMITTER: Campostrini G
PROVIDER: S-EPMC7611409 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Campostrini Giulia G Meraviglia Viviana V Giacomelli Elisa E van Helden Ruben W J RWJ Yiangou Loukia L Davis Richard P RP Bellin Milena M Orlova Valeria V VV Mummery Christine L CL
Nature protocols 20210326 4
Tissue-like structures from human pluripotent stem cells containing multiple cell types are transforming our ability to model and understand human development and disease. Here we describe a protocol to generate cardiomyocytes (CMs), cardiac fibroblasts (CFs) and cardiac endothelial cells (ECs), the three principal cell types in the heart, from human induced pluripotent stem cells (hiPSCs) and combine them in three-dimensional (3D) cardiac microtissues (MTs). We include details of how to differe ...[more]