Dataset Information

Comparative Analysis of Thrombin Calibration Algorithms and Correction for Thrombin-?2macroglobulin Activity.

ABSTRACT:

Background

The thrombin generation (TG) test is useful for characterizing global hemostasis potential, but fluorescence substrate artifacts, such as thrombin-?2macroglobulin (T-?2MG) signal, inner filter effect (IFE), substrate consumption, and calibration algorithms have been suggested as sources of intra- and inter-laboratory variance, which may limit its clinical utility.

Methods

Effects of internal vs. external normalization, IFE and T-?2MG on TG curves in normal plasma supplemented with coagulation factors, thrombomodulin, and tissue factor were studied using the Calibrated Automated Thrombinography (CAT; Diagnostica Stago, Parsippany, NJ, USA) and in-house software.

Results

The various calibration methods demonstrated no significant difference in producing TG curves, nor increased the robustness of the TG assay. Several TG parameters, including thrombin peak height (TPH), produced from internal linear calibration did not differ significantly from uncalibrated TG parameters. Further, TPH values from internal linear and nonlinear calibration with or without T-?2MG correction correlated well with TPH from external calibration. Higher coefficients of variation (CVs) for TPH values were observed in both platelet-free and platelet-rich plasma with added thrombomodulin.

Conclusions

Our work suggests minimal differences between distinct computational approaches toward calibrating and correcting fluorescence signals into TG levels, with most samples returning similar or equivalent TPH results.

SUBMITTER: Chang WC

PROVIDER: S-EPMC7650706 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Publications

Comparative Analysis of Thrombin Calibration Algorithms and Correction for Thrombin-α2macroglobulin Activity.

Chang William C WC Jackson Joseph W JW Machlus Kellie R KR Wolberg Alisa S AS Ovanesov Mikhail V MV

Journal of clinical medicine 20200924 10

<h4>Background</h4>The thrombin generation (TG) test is useful for characterizing global hemostasis potential, but fluorescence substrate artifacts, such as thrombin-α2macroglobulin (T-α2MG) signal, inner filter effect (IFE), substrate consumption, and calibration algorithms have been suggested as sources of intra- and inter-laboratory variance, which may limit its clinical utility.<h4>Methods</h4>Effects of internal vs. external normalization, IFE and T-α2MG on TG curves in normal plasma supple ...[more]

PMID: 32987791

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