Ontology highlight
ABSTRACT:
SUBMITTER: Liu Z
PROVIDER: S-EPMC7655825 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Liu Zhouzerui Z Yuan Jinzhou J Lasorella Anna A Iavarone Antonio A Bruce Jeffrey N JN Canoll Peter P Sims Peter A PA
Scientific reports 20201110 1
Live cell imaging allows direct observation and monitoring of phenotypes that are difficult to infer from transcriptomics. However, existing methods for linking microscopy and single-cell RNA-seq (scRNA-seq) have limited scalability. Here, we describe an upgraded version of Single Cell Optical Phenotyping and Expression (SCOPE-seq2) for combining single-cell imaging and expression profiling, with substantial improvements in throughput, molecular capture efficiency, linking accuracy, and compatib ...[more]