Ontology highlight
ABSTRACT:
SUBMITTER: Yesbolatova A
PROVIDER: S-EPMC7659001 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Yesbolatova Aisha A Saito Yuichiro Y Kitamoto Naomi N Makino-Itou Hatsune H Ajima Rieko R Nakano Risako R Nakaoka Hirofumi H Fukui Kosuke K Gamo Kanae K Tominari Yusuke Y Takeuchi Haruki H Saga Yumiko Y Hayashi Ken-Ichiro KI Kanemaki Masato T MT
Nature communications 20201111 1
Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes it possible to observe an immediate phenotype. However, the current AID system has two major drawbacks: leaky degradation and the requirement for a high dose of auxin. These negative features make it difficult to control precisely the expression level of a protein of interest in living cells and to apply this method to mice. Here, ...[more]