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Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging.

ABSTRACT: G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB1), we discovered periodically repeating clusters of CB1 hotspots within the axons of neurons. We observed these CB1 hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB1 signaling. Furthermore, we found that CB1 hotspot periodicity increased upon CB1 agonist application, and these activated CB1 displayed less dynamic movement compared to non-activated CB1. Our results suggest that CB1 forms periodic hotspots organized by the MPS as a mechanism to increase signaling efficacy upon activation.

SUBMITTER: Li H

PROVIDER: S-EPMC7659323 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Organized cannabinoid receptor distribution in neurons revealed by super-resolution fluorescence imaging.

Li Hui H Yang Jie J Tian Cuiping C Diao Min M Wang Quan Q Zhao Simeng S Li Shanshan S Tan Fangzhi F Hua Tian T Qin Ya Y Lin Chao-Po CP Deska-Gauthier Dylan D Thompson Garth J GJ Zhang Ying Y Shui Wenqing W Liu Zhi-Jie ZJ Wang Tong T Zhong Guisheng G

Nature communications 20201111 1

G-protein-coupled receptors (GPCRs) play important roles in cellular functions. However, their intracellular organization is largely unknown. Through investigation of the cannabinoid receptor 1 (CB1), we discovered periodically repeating clusters of CB1 hotspots within the axons of neurons. We observed these CB1 hotspots interact with the membrane-associated periodic skeleton (MPS) forming a complex crucial in the regulation of CB1 signaling. Furthermo ...[more]

PMID: 33177502

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