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In-Vivo Antidiabetic Activity and In-Silico Mode of Action of LC/MS-MS Identified Flavonoids in Oleaster Leaves.

ABSTRACT: BACKGROUND:Olea europea L. subsp. europaea var. sylvestris (Mill) Lehr (Oleaster) is a wild endemic olive tree indigenous to the Mediterranean region. Olea europea leaves represent a natural reservoir of bioactive molecules that can be used for therapeutic purposes. AIM OF THE STUDY:This work was conducted to study antidiabetic and antihyperglycemic activities of flavonoids from oleaster leaves using alloxan-induced diabetic mice. The mode of action of flavonoids against eight receptors that have a high impact on diabetes management and complication was also investigated using molecular docking. RESULTS:During 28 days of mice treatment with doses 25 and 50 mg/kg b.w, the studied flavonoids managed a severe diabetic state (<450 mg/dL), exhibiting a spectacular antidiabetic and antihyperglycemic activity, and improved mice health status compared to diabetic control. The in-silico mode of action of oleaster flavonoids revealed the inhibition of protein tyrosine phosphatase 1B (PTP1B), Dipeptidyl-peptidase 4 (DPP4), ?-Amylase (AAM), ?-Glucosidase inhibition, Aldose reductase (AldR), Glycogen phosphorylase (GP), and the activation of free fatty acid receptor 1 (FFAR1). CONCLUSION:The findings obtained in the present work indicate that the flavonoids from the oleaster may constitute a safe multi-target remedy to treat diabetes.

SUBMITTER: Mechchate H

PROVIDER: S-EPMC7663640 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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In-Vivo Antidiabetic Activity and In-Silico Mode of Action of LC/MS-MS Identified Flavonoids in Oleaster Leaves.

Mechchate Hamza H Es-Safi Imane I Bourhia Mohammed M Kyrylchuk Andrii A El Moussaoui Abdelfattah A Conte Raffaele R Ullah Riaz R Ezzeldin Essam E Mostafa Gamal A GA Grafov Andriy A Bekkari Hicham H Bousta Dalila D

Molecules (Basel, Switzerland) 20201101 21

<h4>Background</h4><i>Olea europea</i> L. subsp. <i>europaea</i> var. <i>sylvestris</i> (Mill) Lehr (Oleaster) is a wild endemic olive tree indigenous to the Mediterranean region. <i>Olea europea</i> leaves represent a natural reservoir of bioactive molecules that can be used for therapeutic purposes.<h4>Aim of the study</h4>This work was conducted to study antidiabetic and antihyperglycemic activities of flavonoids from oleaster leaves using alloxan-induced diabetic mice. The mode of action of ...[more]

PMID: 33139638

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Dataset Information

In-Vivo Antidiabetic Activity and In-Silico Mode of Action of LC/MS-MS Identified Flavonoids in Oleaster Leaves.

Publications

In-Vivo Antidiabetic Activity and In-Silico Mode of Action of LC/MS-MS Identified Flavonoids in Oleaster Leaves.

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