Dataset Information

New Model for Stacking Monomers in Filamentous Actin from Skeletal Muscles of Oryctolagus cuniculus.

ABSTRACT: To date, some scientific evidence (limited proteolysis, mass spectrometry analysis, electron microscopy (EM)) has accumulated, which indicates that the generally accepted model of double-stranded of filamentous actin (F-actin) organization in eukaryotic cells is not the only one. This entails an ambiguous understanding of many of the key cellular processes in which F-actin is involved. For a detailed understanding of the mechanism of F-actin assembly and actin interaction with its partners, it is necessary to take into account the polymorphism of the structural organization of F-actin at the molecular level. Using electron microscopy, limited proteolysis, mass spectrometry, X-ray diffraction, and structural modeling we demonstrated that F-actin presented in the EM images has no double-stranded organization, the regions of protease resistance are accessible for action of proteases in F-actin models. Based on all data, a new spatial model of filamentous actin is proposed, and the F-actin polymorphism is discussed.

SUBMITTER: Glyakina AV

PROVIDER: S-EPMC7664232 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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New Model for Stacking Monomers in Filamentous Actin from Skeletal Muscles of <i>Oryctolagus cuniculus</i>.

Glyakina Anna V AV Surin Alexey K AK Grishin Sergei Yu SY Selivanova Olga M OM Suvorina Mariya Yu MY Bobyleva Liya G LG Vikhlyantsev Ivan M IM Galzitskaya Oxana V OV

International journal of molecular sciences 20201106 21

To date, some scientific evidence (limited proteolysis, mass spectrometry analysis, electron microscopy (EM)) has accumulated, which indicates that the generally accepted model of double-stranded of filamentous actin (F-actin) organization in eukaryotic cells is not the only one. This entails an ambiguous understanding of many of the key cellular processes in which F-actin is involved. For a detailed understanding of the mechanism of F-actin assembly and actin interaction with its partners, it i ...[more]

PMID: 33171915

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Dataset Information

New Model for Stacking Monomers in Filamentous Actin from Skeletal Muscles of Oryctolagus cuniculus.

Publications

New Model for Stacking Monomers in Filamentous Actin from Skeletal Muscles of <i>Oryctolagus cuniculus</i>.

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