Ontology highlight
ABSTRACT:
SUBMITTER: Cortez FJ
PROVIDER: S-EPMC7665791 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Cortez Felipe de Jesus FJ Gebhart David D Robinson Peter V PV Seftel David D Pourmandi Narges N Owyoung Jordan J Bertozzi Carolyn R CR Wilson Darrell M DM Maahs David M DM Buckingham Bruce A BA Mills John R JR Roforth Matthew M MM Pittock Sean J SJ McKeon Andrew A Page Kara K Wolf Wendy A WA Sanda Srinath S Speake Cate C Greenbaum Carla J CJ Tsai Cheng-Ting CT
PloS one 20201113 11
Islet autoantibodies are predominantly measured by radioassay to facilitate risk assessment and diagnosis of type 1 diabetes. However, the reliance on radioactive components, large sample volumes and limited throughput renders radioassay testing costly and challenging. We developed a multiplex analysis platform based on antibody detection by agglutination-PCR (ADAP) for the sample-sparing measurement of GAD, IA-2 and insulin autoantibodies/antibodies in 1 μL serum. The assay was developed and va ...[more]