Unknown

Dataset Information

0

Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing.

ABSTRACT: A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent and diseased/inflamed lymph nodes were experimentally recapitulated using a flow-based microfluidic perfusion system to assess the effects of physiological flow fields on human metastatic cancer cell adhesion. Results suggest that both altered fluid flow profiles and presentation of adhesive ligands, which are predicted to manifest within the lymph node subcapsular sinus as a result of inflammation-induced remodeling, and the presence of lymph-borne monocytic cells may synergistically contribute to the dynamic extent of cell adhesion in flow relevant to lymph node invasion by cancer and monocytic immune cells during lymphatic metastasis.

SUBMITTER: Birmingham KG 

PROVIDER: S-EPMC7672279 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing.

Birmingham Katherine G KG   O'Melia Meghan J MJ   Bordy Samantha S   Reyes Aguilar David D   El-Reyas Bassel B   Lesinski Gregory G   Thomas Susan N SN  

iScience 20201031 11

A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent and diseased/inflamed lymph nodes were experimentally recapitulated using a flow-based microfluidic perfusion system  ...[more]

Similar Datasets

Unknown Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis.
| S-EPMC6930078 | biostudies-literature
Unknown Immunomodulatory Responses of Subcapsular Sinus Floor Lymphatic Endothelial Cells in Tumor-Draining Lymph Nodes.
| S-EPMC9330828 | biostudies-literature
Unknown Infection-induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus.
| S-EPMC3442246 | biostudies-literature
Unknown Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift.
| S-EPMC9072412 | biostudies-literature
Unknown Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis.
| S-EPMC6529739 | biostudies-literature
Unknown Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche.
| S-EPMC6697131 | biostudies-literature
Unknown Tolerogenic properties of lymphatic endothelial cells are controlled by the lymph node microenvironment.
| S-EPMC3913631 | biostudies-literature
Transcriptomics Gene expression analysis of mouse lymphatic endothelial cells (LECs) from the subcapsular sinus (SS, afferent) and lymphatic sinus (LS, efferent)
2016-08-24 | GSE68371 | GEO
Transcriptomics Gene expression analysis of mouse lymphatic endothelial cells (LECs) from the subcapsular sinus (SS, afferent) and lymphatic sinus (LS, efferent)
2016-08-24 | E-GEOD-68371 | biostudies-arrayexpress
Unknown Harnessing the lymph node microenvironment.
| S-EPMC6309445 | biostudies-literature