Ontology highlight
ABSTRACT:
SUBMITTER: Liebel M
PROVIDER: S-EPMC7673696 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Science advances 20201104 45
Fluorescence microscopy is the method of choice in biology for its molecular specificity and super-resolution capabilities. However, it is limited to a narrow <i>z</i> range around one observation plane. Here, we report an imaging approach that recovers the full electric field of fluorescent light with single-molecule sensitivity. We expand the principle of digital holography to fast fluorescent detection by eliminating the need for phase cycling and enable three-dimensional (3D) tracking of ind ...[more]