A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.

ABSTRACT: Diffusion is a major molecular transport mechanism in biological systems. Quantifying direction-dependent (i.e., anisotropic) diffusion is vitally important to depicting how the three-dimensional (3D) tissue structure and composition affect the biochemical environment, and thus define tissue functions. However, a tool for noninvasively measuring the 3D anisotropic extracellular diffusion of biorelevant molecules is not yet available. Here, we present light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP), which noninvasively determines 3D diffusion tensors of various biomolecules with diffusivities up to 51 µm² s^-1, reaching the physiological diffusivity range in most biological systems. Using cornea as an example, LiFT-FRAP

SUBMITTER: Chen P 

PROVIDER: S-EPMC7997923 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature