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Imidazole propionate is increased in diabetes and associated with dietary patterns and altered microbial ecology.

ABSTRACT: Microbiota-host-diet interactions contribute to the development of metabolic diseases. Imidazole propionate is a novel microbially produced metabolite from histidine, which impairs glucose metabolism. Here, we show that subjects with prediabetes and diabetes in the MetaCardis cohort from three European countries have elevated serum imidazole propionate levels. Furthermore, imidazole propionate levels were increased in subjects with low bacterial gene richness and Bacteroides 2 enterotype, which have previously been associated with obesity. The Bacteroides 2 enterotype was also associated with increased abundance of the genes involved in imidazole propionate biosynthesis from dietary histidine. Since patients and controls did not differ in their histidine dietary intake, the elevated levels of imidazole propionate in type 2 diabetes likely reflects altered microbial metabolism of histidine, rather than histidine intake per se. Thus the microbiota may contribute to type 2 diabetes by generating imidazole propionate that can modulate host inflammation and metabolism.

SUBMITTER: Molinaro A

PROVIDER: S-EPMC7676231 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Imidazole propionate is increased in diabetes and associated with dietary patterns and altered microbial ecology.

Molinaro Antonio A Bel Lassen Pierre P Henricsson Marcus M Wu Hao H Adriouch Solia S Belda Eugeni E Chakaroun Rima R Nielsen Trine T Bergh Per-Olof PO Rouault Christine C André Sébastien S Marquet Florian F Andreelli Fabrizio F Salem Joe-Elie JE Assmann Karen K Bastard Jean-Philippe JP Forslund Sofia S Le Chatelier Emmanuelle E Falony Gwen G Pons Nicolas N Prifti Edi E Quinquis Benoit B Roume Hugo H Vieira-Silva Sara S Hansen Tue H TH Pedersen Helle Krogh HK Lewinter Christian C Sønderskov Nadja B NB Køber Lars L Vestergaard Henrik H Hansen Torben T Zucker Jean-Daniel JD Galan Pilar P Dumas Marc-Emmanuel ME Raes Jeroen J Oppert Jean-Michel JM Letunic Ivica I Nielsen Jens J Bork Peer P Ehrlich S Dusko SD Stumvoll Michael M Pedersen Oluf O Aron-Wisnewsky Judith J Clément Karine K Bäckhed Fredrik F

Nature communications 20201118 1

Microbiota-host-diet interactions contribute to the development of metabolic diseases. Imidazole propionate is a novel microbially produced metabolite from histidine, which impairs glucose metabolism. Here, we show that subjects with prediabetes and diabetes in the MetaCardis cohort from three European countries have elevated serum imidazole propionate levels. Furthermore, imidazole propionate levels were increased in subjects with low bacterial gene richness and Bacteroides 2 enterotype, which ...[more]

PMID: 33208748

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Project description:Dietary supplementation with calcium propionate can effectively alleviate negative energy balance and hypocalcemia of dairy cows in early lactation. The objective of this study was to investigate the effects of calcium propionate feeding levels on the immune function, liver function, and fecal microbial composition of dairy cows in early lactation. Thirty-two multiparous Holstein cows were randomly assigned to four treatments after calving. Treatments were a basal diet plus 0, 200, 350, and 500 g calcium propionate per cow per day throughout a 5-week trial period. Cows were milked three times a day, and blood was sampled to measure immune function and liver function on d 7, 21, and 35. The rectal contents were sampled and collected on d 35 to analyze the microbial composition using 16S rRNA gene sequencing. The results indicated that increasing amounts of calcium propionate did not affected the serum concentrations of total protein, IgG, IgM, and calcium, but the concentrations of albumin and IgA changed quadratically. With the increase of calcium propionate, the activity of serum alanine transaminase and aspartate aminotransferase increased linearly, in contrast, the activity of alkaline phosphatase decreased linearly. Moreover, dietary supplementation with increasing levels of calcium propionate tended to quadratically decrease the relative abundance of Firmicutes while quadratically increased the abundance of Bacteroidetes, and consequently linearly decreased the Firmicutes/Bacteroidetes ratio in the rectal microbiota. Additionally, the supplementation of calcium propionate increased the relative abundances of Ruminococcaceae_UCG-005 and Prevotellaceae_UCG-004 linearly, and Ruminococcaceae_UCG-014 quadratically, but decreased the relative abundances of Lachnospiraceae_NK3A20_group and Family_XIII_AD3011_group quadratically. Compared with the CON group, the calcium propionate supplementation significantly decreased the relative abundance of Acetitomaculum but increased the abundances of Rikenellaceae_RC9_gut_group and Alistipes. In summary, these results suggested that the supplementation of calcium propionate to dairy cows in early lactation could beneficially alter the rectal microbiota.

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Imidazole propionate is increased in diabetes and associated with dietary patterns and altered microbial ecology.

Publications

Imidazole propionate is increased in diabetes and associated with dietary patterns and altered microbial ecology.

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