Ontology highlight
ABSTRACT:
SUBMITTER: Bohm MS
PROVIDER: S-EPMC7677229 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Bohm Margaret S MS Dame Michael K MK Boyd Joseph J Su Kevin K Wu Angeline A Attili Durga D Chu Vi V Colacino Justin A JA Spence Jason R JR
Frontiers in cell and developmental biology 20201106
Cell line authentication is critical for preventing the use of mixed or misidentified cell lines in research. Current efforts include short tandem repeat (STR) analysis and PCR-based assays to detect mixed species cultures. Using PCR analysis with mouse-specific primers, we identified contaminating mouse DNA in growth factor conditioned medium (CM) derived from the L-WRN cell line (L-WRN CM), as well as in human organoid cultures maintained in the L-WRN CM. DNA isolated from L-WRN CM matched the ...[more]