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Amyloid ? aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus.

ABSTRACT: Six new triterpene saponins (1-5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer's disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (A?) aggregation using thioflavin-T assay, and triterpenes as an aglycone moiety and an alkaline hydrolysate of the saponins were also evaluated. One saponin, stenoside A (7), exhibited inhibitory activity related to A? aggregation and its degree of A? aggregation was 40.6% at 100 ?M.

SUBMITTER: Fujihara K

PROVIDER: S-EPMC7684148 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus.

Fujihara Koji K Shimoyama Takuya T Kawazu Ryo R Sasaki Hiroaki H Koyama Kiyotaka K Takahashi Kunio K Kinoshita Kaoru K

Journal of natural medicines 20201124 2

Six new triterpene saponins (1-5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer's disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (Aβ) aggregation using thioflavin-T assay, and triterpenes as an agly ...[more]

PMID: 33231837

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