Comparison of methylation capture sequencing and Infinium MethylationEPIC array in peripheral blood mononuclear cells.

ABSTRACT:

Background

Epigenome-wide association studies (EWAS) have been widely applied to identify methylation CpG sites associated with human disease. To date, the Infinium MethylationEPIC array (EPIC) is commonly used for high-throughput DNA methylation profiling. However, the EPIC array covers only 30% of the human methylome. Methylation Capture bisulfite sequencing (MC-seq) captures target regions of methylome and has advantages of extensive coverage in the methylome at an affordable price.

Methods

Epigenome-wide DNA methylation in four peripheral blood mononuclear cell samples was profiled by using SureSelectXT Methyl-Seq for MC-seq and EPIC platforms separately. CpG site-based reproducibility of MC-seq was assessed with DNA sample inputs ranging in quantity of high (> 1000 ng),

SUBMITTER: Shu C 

PROVIDER: S-EPMC7684759 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature