Dataset Information

The complete mitochondrial genome of the breed Ningbo brown-marbled grouper (Epinephelus fuscoguttatus).

ABSTRACT: We used NGS technologies to resequence the complete mitochondrial genome of (Epinephelus fuscoguttatus). The complete mitochondrial genome of the breed Ningbo brown-marbled grouper is a 16,373?bp circular molecule, which contains 37 typical mitochondrial genes (13 protein-coding genes, two rRNAs, and 22 tRNAs) and a D-loop. Its gene arrangement pattern is the same as the accession no. KP013758. Comparing to accession no. KP013758 sequence, the D-loop region is much shorter. ATG is the most common start codon, and TAA is the most common stop codons; the 12S rRNA and 16S rRNA are located between the tRNA^Phe and tRNA^Leu genes and are separated by the tRNA^Val gene. Phylogenetic trees indicated E. fuscoguttatus has close relative with Epinephelus coioides and Epinephelus malabaricus.

SUBMITTER: Liu G

PROVIDER: S-EPMC7687516 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome of the breed Ningbo brown-marbled grouper (<i>Epinephelus fuscoguttatus</i>).

Liu Gang G Gong Zhizhong Z Li Qingyue Q

Mitochondrial DNA. Part B, Resources 20190710 2

We used NGS technologies to resequence the complete mitochondrial genome of (<i>Epinephelus fuscoguttatus</i>). The complete mitochondrial genome of the breed Ningbo brown-marbled grouper is a 16,373 bp circular molecule, which contains 37 typical mitochondrial genes (13 protein-coding genes, two rRNAs, and 22 tRNAs) and a D-loop. Its gene arrangement pattern is the same as the accession no. KP013758. Comparing to accession no. KP013758 sequence, the D-loop region is much shorter. ATG is the mos ...[more]

PMID: 33365430

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Project description:As an important protein source, soybean products can cause intestinal inflammation and injury in many animals including human beings, particularly infants and juvenile individuals. Research in this field has been performed for terrestrial animals and fish, but still lacks integrity and systematicness. In this study, the main biological processes in the intestinal tract of marine fish juvenile pearl gentian grouper in the state of soybean meal-induced enteritis (SBMIE) were analyzed. A total of 720 groupers with an approximate initial weight of 12.5 g were randomly divided into three groups: the fish meal (FM) control group, the 20% SBM group (SBM20), and the SBM40 group (n = 4). Three iso-nitrogenous and iso-lipidic diets were prepared and fed to fish for 10 weeks. Each barrel contained a water volume of about 1 m3 in and was exposed to natural light and temperature. Results indicated that the growth and physiology of groupers fed with SBM were significantly negatively affected, with the gene expressions of intestinal structural protein abnormal. 16SrDNA high-throughput sequencing showed that the intestinal microflora played an important role in the pathogenesis of pearl gentian grouper SBMIE, which may activate a variety of pathogen pattern recognition receptors, such as toll-like receptors (TLRs), RIG-I-like receptors, and nod-like receptors. Transcriptome analysis revealed that changes of the SBMIE signaling pathway in pearl gentian groupers were conservative to some extent than that of terrestrial animals and freshwater fish. Moreover, the TLRs-nuclear factor kappa-B signaling pathway becomes activated, which played an important role in SBMIE. Meanwhile, the signal pathways related to nutrient absorption and metabolism were generally inhibited. Metabolomics analysis showed that isoflavones and saponins accounted for a large proportion in the potential biomarkers of pearl gentian grouper SBMIE, and most of the biomarkers had significantly positive or negative correlations with each other; 56 metabolites were exchanged between intestinal tissues and contents, which may play an important role in the development of enteritis, including unsaturated fatty acids, organic acids, amino acids, vitamins, small peptides, and nucleotides, etc. These results provide a basic theoretical reference for solving the intestinal issues of fish SBMIE and research of inflammatory bowel disease in mammals.

Dataset Information

The complete mitochondrial genome of the breed Ningbo brown-marbled grouper (Epinephelus fuscoguttatus).

Publications

The complete mitochondrial genome of the breed Ningbo brown-marbled grouper (<i>Epinephelus fuscoguttatus</i>).

Similar Datasets

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