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Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation.

ABSTRACT:

Background

Ex vivo production of hematopoietic stem/precursor cells (HSPCs) represents a promising versatile approach for blood disorders.

Methods

To derive definitive HSPCs from human embryonic stem cells (ESCs), we differentiated mesodermally specified embryoid bodies (EBs) on gelatin-coated plates in serum/feeder-free conditions.

Results

Seven-day EB maturation followed by an 8-day differentiation period on OP9 cells provided the highest number of definitive (CD34+ CD235a-, 69%, p?ConclusionsIn summary, definitive HSPCs can be derived from ESCs through a dynamic cellular process from an organoid-like structure, where erythroid progeny are capable of producing adult hemoglobin and lymphoid progeny shows a diverse TCR repertoire.

SUBMITTER: Demirci S

PROVIDER: S-EPMC7688003 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation.

Demirci Selami S Haro-Mora Juan J JJ Leonard Alexis A Drysdale Claire C Malide Daniela D Keyvanfar Keyvan K Essawi Khaled K Vizcardo Raul R Tamaoki Naritaka N Restifo Nicholas P NP Tisdale John F JF Uchida Naoya N

Stem cell research & therapy 20201124 1

<h4>Background</h4>Ex vivo production of hematopoietic stem/precursor cells (HSPCs) represents a promising versatile approach for blood disorders.<h4>Methods</h4>To derive definitive HSPCs from human embryonic stem cells (ESCs), we differentiated mesodermally specified embryoid bodies (EBs) on gelatin-coated plates in serum/feeder-free conditions.<h4>Results</h4>Seven-day EB maturation followed by an 8-day differentiation period on OP9 cells provided the highest number of definitive (CD34+ CD235 ...[more]

PMID: 33234163

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