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Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells.


ABSTRACT: Hematopoietic stem cells (HSCs) replenish all types of blood cells. It is debating whether HSCs in adults solely originate from the aorta-gonad-mesonephros (AGM) region, more specifically, the dorsal aorta, during embryogenesis. Here, we report that somite hematopoiesis, a previously unwitnessed hematopoiesis, can generate definitive HSCs (dHSCs) in zebrafish. By transgenic lineage tracing, we found that a subset of cells within the forming somites emigrate ventromedially and mix with lateral plate mesoderm-derived primitive hematopoietic cells before the blood circulation starts. These somite-derived hematopoietic precursors and stem cells (sHPSCs) subsequently enter the circulation and colonize the kidney of larvae and adults. RNA-seq analysis reveals that sHPSCs express hematopoietic genes with sustained expression of many muscle/skeletal genes. Embryonic sHPSCs transplanted into wild-type embryos expand during growth and survive for life time with differentiation into various hematopoietic lineages, indicating self-renewal and multipotency features. Therefore, the embryonic origin of dHSCs in adults is not restricted to the AGM.

SUBMITTER: Qiu J 

PROVIDER: S-EPMC4991667 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells.

Qiu Juhui J   Fan Xiaoying X   Wang Yixia Y   Jin Hongbin H   Song Yixiao Y   Han Yang Y   Huang Shenghong S   Meng Yaping Y   Tang Fuchou F   Meng Anming A  

Journal of molecular cell biology 20160601 4


Hematopoietic stem cells (HSCs) replenish all types of blood cells. It is debating whether HSCs in adults solely originate from the aorta-gonad-mesonephros (AGM) region, more specifically, the dorsal aorta, during embryogenesis. Here, we report that somite hematopoiesis, a previously unwitnessed hematopoiesis, can generate definitive HSCs (dHSCs) in zebrafish. By transgenic lineage tracing, we found that a subset of cells within the forming somites emigrate ventromedially and mix with lateral pl  ...[more]

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