Unknown

Dataset Information

0

Deterministic droplet coding via acoustofluidics.


ABSTRACT: Droplet microfluidics has become an indispensable tool for biomedical research and lab-on-a-chip applications owing to its unprecedented throughput, precision, and cost-effectiveness. Although droplets can be generated and screened in a high-throughput manner, the inability to label the inordinate amounts of droplets hinders identifying the individual droplets after generation. Herein, we demonstrate an acoustofluidic platform that enables on-demand, real-time dispensing, and deterministic coding of droplets based on their volumes. By dynamically splitting the aqueous flow using an oil jet triggered by focused traveling surface acoustic waves, a sequence of droplets with deterministic volumes can be continuously dispensed at a throughput of 100 Hz. These sequences encode barcoding information through the combination of various droplet lengths. As a proof-of-concept, we encoded droplet sequences into end-to-end packages (e.g., a series of 50 droplets), which consisted of an address barcode with binary volumetric combinations and a sample package with consistent volumes for hosting analytes. This acoustofluidics-based, deterministic droplet coding technique enables the tagging of droplets with high capacity and high error-tolerance, and can potentially benefit various applications involving single cell phenotyping and multiplexed screening.

SUBMITTER: Zhang P 

PROVIDER: S-EPMC7688411 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5536981 | biostudies-literature
| S-EPMC6626638 | biostudies-literature
| S-EPMC7239761 | biostudies-literature
| S-EPMC6934416 | biostudies-literature
| S-EPMC6189727 | biostudies-literature
| S-EPMC7902458 | biostudies-literature
| S-EPMC5385560 | biostudies-literature
| S-EPMC5897416 | biostudies-literature
| S-EPMC9695966 | biostudies-literature
| S-EPMC6062562 | biostudies-literature