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CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites.

ABSTRACT: CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in a multi-well cell culture plate to profile RNA polymerase II. Long (>?270 bp) DNA fragments released by CUT&RUN corresponded to the bimodal peak around the transcription start sites, as previously seen with chromatin immunoprecipitation. However, we found that short (

SUBMITTER: Miura M

PROVIDER: S-EPMC7691310 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites.

Miura Michi M Chen Honglin H

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 20201018 3-4

CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in a multi-well cell culture plate to profile RNA polymerase II. Long (> 270 bp) DNA fragments released by CUT&RUN corresponded to the bimodal peak around the transcription start sites, as previously seen with chromatin immunoprecipit ...[more]

PMID: 33070289

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