Ontology highlight
ABSTRACT:
SUBMITTER: Miura M
PROVIDER: S-EPMC7691310 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 20201018 3-4
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in a multi-well cell culture plate to profile RNA polymerase II. Long (> 270 bp) DNA fragments released by CUT&RUN corresponded to the bimodal peak around the transcription start sites, as previously seen with chromatin immunoprecipit ...[more]